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A Simple SEM (Scanning Electron Microscope) Preparation Protocol for Tardigrades

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Mitchell_&_Miller,_2008_(2070)_Page_1

Tardigrades are animals that lend themselves to Scanning
Electron Microscopy (SEM), yet the literature is
vague about the process of preparing of the animals for
the procedure. A simple, step-by-step protocol for the
preparation of tardigrades for SEM is provided. The
method includes the construction of a processing chamber,
the handling of specimens, the steps for dehydration,
critical point drying, and sputter coating. Finally,
tips for mounting the animals on stubs are presented.
[J PA Acad Sci 81(2/3): 86-90, 2008] Keywords: Critical Point Drying, dehydration, sputter
coating, SEM

~Ella

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